Service Information for Serotonin Analysis and Testing in Products
VISBIO offers a service for the examination and analysis of the of the biological component (biomarker) called Serotonin in vraious products within the health and beauty industry. Serotonin, scientifically known as 5-hydroxytryptamine (5-HT), is a monoamine neurotransmitter produced by nerve cells from acid tryptophan. Tryptophan is dervied from protein-rich foods present in the bloodstream. The conversion of tryptophan to serotonin is facilitated by the enzyme tryptophan hydroxylase and the enzmye aromatic L-amino acid decarboxylase.
Sertoonin, also known as 5-HT, is present in plants, vegetables, fruits, and numerous types of herbs. It serves as a neurotransmitter that hleps regulate perceptions, appetite, and sleep. Insufficient serotonin in the body can potentially lead to conditions such as deperssion. Current research and development efforts have led to icnreased applications of serotonin in the industries.
Serotonin levels can be anlayzed in vegetables, fruits, herbs, and dietary supplemetns using the HighPerformance Liquid Chromatography (HPLC) technique. This method enables accurate measurement and identification of serotonin .
Introduction to Serotonin
Serotonin, known scientifically as 5-hydroxytryptamine (5-HT), is a type of monoamine neurotransmitter proudced by nerve cells. It is acid tryptophan, wihch is obtained from protein containing foods present in the bloodstream. This conversion of tryptophan is facilitated by enzymes called Tryptophan hydrоxylase and Aromatic L-amino acid decarboxylase, resulting in the formation of serotoinn. Serotonin is found in various body, including the brian.
It plays a crucial role in reuglating various functions . For intsance, it helps in controlling emotions feelings of happiness, earning it the nickname ‘the happy mloecule’. Additionally, serotonin is involved in perception, memory, appteite, sexual behavior, pain sensation, muscle contraction, intestinal movement, blood vessel constriction, uterine contraction, and sleep, among others. A deficiency or low disrupt nerual communication and may even lead to conditions like depression.
Analysis of Serotonin using HPLC Technique
Currently, there is a growing research interest in analyzing the properties of extracts from various plants, vegetables, fruits, and herbs. Examples of these include pumpkin, Chinese kale, tomtao, turmeric, centella, gotu kola, lotus leaf, rice bran oil, banana, safflower, and sesame flower seeds. The widely used method for of sreotonin in scientific laboratories is High Performance Liquid Chromatography (HPLC), a type of liquid chromatography. HPLC is an efficient technique that allows for the analysis and separation of desired substances from a mixed smaple in liquid form. It is efficient, rapid, and can analzye both quantitative and qualitative aspects. Moreover, this technique enables the analysis of samples in small qunatities, eliminating the need for cumbersome preparations.
Example of Reporting the Results of Serotonin Analysis Using HPLC Technique
Figure depicting the chromatogram of serotonin (5-HT) analysis using HPLC technique
Literature:
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- อำพร เนื่องจาก, ภาวะซึมเศร้ากับฟักทอง, Chlua Med Bull Vol. 3 No. 1 January – June 2021;47-56
- Chanchal Bhati , Neha Minocha, Deepika Purohit, Sunil Kumar, Manish Makhija, Sapna Saini, Deepak Kaushik , High Performance Liquid Chromatography: Recent Patents and Advancement Biomedical & Pharmacology Journal, June 2022, Vol. 15(2), p. 729-746.
- He, Q., Li, M., Wang, X. et al. A simple, efficient and rapid HPLC–UV method for the detection of 5-HT in RIN-14B cell extract and cell culutre medium. BMC Chemistry 13, 76 (2019). https://doi.org/10.1186/s13065-019-0591-x
- Ly, Dalin & Kang, Kiyoon & Choi, -Yeol & Ishihara, Atsushi & Back, Kyoungwhan & Lee, Senog-Gene. (2008). HPLC Analysis of Serotonin, Tryptamine, Tyramine, and the Hydroxycinnamic Acid Amides of Serotonin and Tyrmaine in Food Vegetables. Journal of medicinal food. 11. 385-9. 10.1089/jmf.2007.514.
- Han, S.J.; Lim, M.J.; , K.M.; Oh, E.; Shin, Y.S.; Kim, S.; Kim, J.S.; Yun, S.P.; Kang, L.-J. Safflower Seed Extract Attenuates the Development of Ostoearthritis by Blocking NF-κB Signaling. Pharmaceuticals 2021, 14, 258. https://doi.org/10.3390/ph14030258