Information for analyzing the quantity of Andrographolide using HPLC technique

VISBIO offers services for the examination and analysis of biomarkers, specifically Andrographolide, using the HPLC (High-Performance Liquid Chromatography) technique. This service is available for all health and beauty products in the industry. Andrographolide is a type of lactone compound and is the main active ingredient found in the medicinal herb “Andrographis paniculata (Burm. F.) Wall. ex Nees,” also known as “Fa Talai Jone” in Thai. It is considered a potent and safe herbal component and has been listed in the National Essential Drug List since 1999.

Andrographolide, along with two other important compounds, Neo-Andrographolide and 14-deoxyandrographolide, are the bioactive substances in the “Fa Thalai Chon” herb. These compounds are Lactones and have various medicinal properties. Research has shown that “Fa Thalai Chon” possesses antipyretic, anti-inflammatory, and analgesic effects due to its diverse bioactive components, such as Diterpenoids, Flavonoids, and Polyphenols. It exhibits anti-inflammatory and anti-infection properties, stimulates the immune system, and helps alleviate symptoms of common colds, liver-related ailments, infections, and various skin conditions.

Andrographis paniculata (Fa thalai chon)

Andrographis paniculata, commonly known as “King of Bitters” or “Green Chiretta,” is a native medicinal herb found in the eastern regions of Asia, such as India, China, Sri Lanka, and Thailand. It is widely used as a traditional herbal remedy for various ailments, especially for treating severe colds and respiratory illnesses. In Thailand, Andrographis paniculata is included in the National List of Essential Medicines under the category of drugs used for digestive and respiratory disorders.

The leaves and underground stems of Andrographis paniculata are commonly used to make medicinal preparations. It is known for its dark green leaves and is scientifically classified under the Acanthaceae family. It is also known by various names in Thai, including “Pong Siang Fah Ta-Lai,” “Ngha Gun Ngoo,” “Nam Lai Pung Porn,” “Mek Ta Lai,” and “Fah Sa Tarn”.

The medicinal properties of Andrographis paniculata

The medicinal properties of Andrographis paniculata are attributed to three important compounds known as Lactones. These compounds are “Andrographolide,” “Neo-Andrographolide,” and “14-deoxy-andrographolide.” These substances are responsible for the herb’s medicinal effects.

Andrographis paniculata (Fa Thalai Chon), Herbal medicine in Thai traditional medicine

Andrographis paniculata  (Fa Thalai Chon) is a valuable herbal medicine in Thai traditional medicine. Thai herbs are a cultural heritage that holds significant value and has been passed down from generation to generation. They possess medicinal properties and are commonly used as ingredients in cooking, cosmetics, and traditional medicine. Andrographis paniculata is a bitter-tasting local herb that has been traditionally used to treat colds since ancient times.

This herb contains a variety of biologically active substances such as Diterpenoids, Flavonoids, and Polyphenols, which have anti-inflammatory and anti-infective properties. It can stimulate the immune system and alleviate symptoms of colds or diseases related to the liver (e.g., fatty liver, hepatitis caused by medication), infections (syphilis, pneumonia, tuberculosis, abscesses), HIV/AIDS, or other skin conditions.

There are various forms of using Andrographis paniculata

  1. For a simple infusion, take one handful of mature leaves, crush them, and mix with alcohol and water. Afterward, strain and drink the liquid. The remaining pulp can be used to apply to wounds by using it as a poultice.
  2. For a decoction, use fresh leaves and branches, wash them thoroughly, and cut them into small pieces. Boil them in water for 10-15 minutes, then drink the decoction before meals to relieve a sore throat.
  3. For a maceration with alcohol, pound the leaves of Andrographis paniculata and put them in a bottle filled with alcohol. Close the bottle tightly and shake it. Drink this mixture 3-4 times a day before meals.
  4. For preparing pills, grind the leaves and branches into a fine powder and mix them with honey. Shape the mixture into small pills and take them before bedtime and before meals.
  5. Ready-made capsules are also available. The powdered leaves and stems are encapsulated for convenient consumption. Capsules can be taken before meals and before bedtime. They are preferred by many due to their ability to mask the bitter taste.

Andrographis paniculata: Herbal Remedy More Than Just COVID-19 Treatment

Andrographis paniculata, a bitter-tasting traditional Thai herb, has been widely used for treating colds since ancient times. With the recent COVID-19 pandemic, it has also been employed to alleviate symptoms related to the virus. These symptoms bear similarities to common colds but are more severe. As a result, Andrographis paniculata has been considered as an alternative option to help ease these particular symptoms.

This herbal remedy, officially included in the National Essential Medicines List, contains a significant compound called “Andrographolide.” This compound has properties that can inhibit the virus’s growth, reduce inflammation, and stimulate the immune system. Thus, it addresses the root causes of respiratory infections.

The Department of Thai Traditional and Alternative Medicine has endorsed the acceptance and use of The Bandit’s Bane alongside conventional medical treatments. Research studies on patients with mild COVID-19 symptoms, totaling 309 cases, have shown that the extract from Andrographis paniculata, containing

Andrographolide, could inhibit the virus’s replication and reduce the severity of the disease. However, it was not found to be effective in preventing COVID-19 infection.

Development of products from herbal extracts, Fa Thalai Chon (Andrographis paniculata), for health and beauty purposes.

Currently, herbal products have gained widespread popularity in various forms, including herbal medicine, dietary supplements, and cosmetics, with a growing demand in the global market. Although Thailand has a rich diversity of herbal plants, it imports more herbal products than it exports. This is because Thais tend to prefer products from foreign countries, including those made from herbs.

While foreign countries show great interest in Thai herbs, Thailand has limited information and knowledge about its own herbal resources. Most of the herbal products exported from Thailand are essential oils or various fragrances. Despite the long-standing use of Thai herbs in traditional medicine, skincare products, and fragrances, it is unfortunate that no Thai herbal product has made its way into the global market.

Thailand has several herbal species with cosmetic properties that could be developed into competitive products in the global market. About ten interesting herbs for cosmetic development include “Wan Haeng Chorakhe” (Andrographis paniculata), “Kaminchan” (Curcuma aeruginosa Roxb.), “Kwaokrua Kao” (Pueraria mirifica),”Maawn” (Cucumis melo), “Chumhet Thet” (Tremella fuciformis), “Makham Pom” (Syzygium cuminii), “Bua Bok”(Nymphaea lotus), “Prik Thai Dam” (Piper retrofractum), “Plai” (Zingiber cassumunar), and “Fa Thalai Chon” (Andrographis paniculata). Fa Thalai Chon contains anti-inflammatory properties and could be used in products for skin protection, particularly in sunscreens and skin-whitening products, potentially replacing the use of chamomile extracts from other countries.

Example of Andrographolide Analysis Report Using HPLC Technique


  • ธนโชติ ธรรมชาติ. “ฟ้าทะลายโจร” ธนโชติ ธรรมชาติ หน่วยวิเคราะห์ วิจัยพฤกษเคมี ศูนย์ปฏิบัติการวิจัยและเรือนปลูกพืชทดลอง มหาวิทยาลัยเกษตรศาสตร์ วิทยาเขตกำแพงแสน
  • “ยาฟ้าทะลายโจร” กรมการแพทย์แผนไทยและการแพทย์ทางเลือก “ยาฟ้าทะลายโจร”
  • “ประกาศคณะกรรมการพัฒนาระบบยาแห่งชาติ” เรื่อง บัญชียาหลักแห่งชาติด้านสมุนไพร พ.ศ. 2564 เล่ม 139 ตอนพิเศษ 41 ง หน้า 47 วันที่ 18 กุมภาพันธ์ 2564
  • “เผยสมุนไพรไทยเจ๋ง เพิ่มผิวขาวเนียน-ลดแก่ แก้หงอก” เผยแพร่: 15 ส.ค. 2549 11:51 โดย: MGR Online
  • “สรรพคุณ ฟ้าทะลายโจร” การแพทย์แผนไทยประยุกต์ศิริราช
  • รองศาสตราจารย์ ดร.ภญ.นพมาศ สนทรเจริญนนท์ “ฟ้าทะลายโจร สามารถนํามา ใช้ในสถานการณ์ COVID-19 ระบาด ได้จรงหรือ” ประธานวิทยาลัยเภสัชกรรมสมนไพรแหงประเทศไทย ทีปรกษาสานกงานขอมลสมนไพร คณะเภสัชศาสตร์ มหิดล
  • เรื่องน่ารู้เกี่ยวกับ “ฟ้าทะลายโจร” Chulabhorn Research Institute “”
  • มาตรฐานสมุนไพรไทยเล่มที่ 1 ฟ้าทะลายโจร โรงพิมพ์องค์การสงเคราะห์ทหารผ่านศึก กรุงเทพฯ. 2542.
  • เภสัชกร รวีภัทร์ อนรรฆเมธี “ฟ้าทะลายโจรกับการรักษา COVID-19” งานเภสัชกรรมคลินิก ฝ่ายเภสัชกรรม คณะแพทยศาสตร์โรงพยาบาลรามาธิบดี มหาวิทยาลัยมหิดล ฉบับที่ 42 เดือนตุลาคม 2564 Rama RDU
  • นสภ.พุทธินันท์ อรพินท์พิศุทธิ์ นสภ.นวลกนก กุลมโนชญ์ และภก.อนวัช มิตรประทาน “การใช้สมุนไพรฟ้าทะลายโจรในการรักษา COVID-19 “ สถานเสาวภา สภากาชาดไทย Arunkumar Phurailatpam, Ashok Kumar Bishoyi and Satyabrata Maiti, 2014, An assessment of andrographolide production in Andrographispaniculata grown in different agro-climatic locations, African Journal of Agricultural, Vol. 8(48), pp. 6101-6109.